A Study of Meticillin Resistant Pattern on Clinical Isolates of Staphylococcus aureus in Tertiary Care Hospitals of Pokhara

Authors: Suresh Jaiswal,Anching Thapa,Gaurab Mali,Sabina Magar,Sanjaya Gurung, Sweekriti Shakya,Bishnu Raj Tiwari

Meticillin-resistant Staphylococcus aureus (MRSA) has become one of the well-known etiologic agents for a wide variety of infections in both hospital and community settings. It is also a growing threat to the immunocompromised as well as to the general public. A total of 98 S. aureus isolates from 450 different human clinical specimens comprising pus, nasal swab, blood, urine and sputum were obtained at two tertiary care hospitals of Pokhara; Manipal Teaching Hospital (MTH) and Western Regional Hospital (WRH).

Those isolates were then screened for meticillin resistance by the Kirby Bauer disc diffusion technique following aseptic procedures in Microbiology laboratory, WRH. Antibiotic susceptibility pattern of Meticillin sensitive S. aureus (MSSA) and Meticillin resistant S. aureus (MRSA) were studied by using antibiotic discs like cefoxitin (30mcg), oxacillin (1mcg), vancomycin (30mcg) and gentamicin (10mcg). 72.4% of the isolates were found to be MRSA while 27.6% were MSSA. Among them, very high resistance levels (87.8%) and (74.5%) were detected against oxacillin and cefoxitin while gentamicin and vancomycin recorded the least resistance levels i.e (25.5%) and (5.1%) respectively. High percentage of meticillin resistant isolates and occurence of vancomycin resistance among them which may refer to irrational use of antimicrobial agent, thus, necessitate implementation of good strategies for control of infection and use of antibiotics. Outcome of this study emphasizes the need for constant monitoring on the prevalence of MRSA and to help clinicians/doctors in the effective management and treatment of infections caused by S.aureus.[Full Article]

KEYWORDS: MRSA, Antimicrobial Susceptibility, Meticillin, Cefoxitin, Oxacillin, Vancomycin

Published in : BMR Microbiology

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