Phytochemical assessment, in vitro antimicrobial and antioxidant activities of Acacia hockii De Wild

Authors: Latifou Lagnika, Mounirou Tchatchedre, Abdou Madjid O. Amoussa, Kamal Latoundji, Ambaliou Sanni

Aqueous and ethanolic extracts of leafy stems of Acacia hockii harvested in Tanguiéta, were examined for antimicrobial and antioxidant activities. Phytochemical screening of A. hockii was performed using two complementary methods: tube test and thin layer chromatography method. Antimicrobial activities of aqueous and ethanolic extracts were investigated against four Gram Positive and two Gram negative strains bacteria and three species of Aspergillus. The microdilution and agar diffusion methods were used to evaluate antibacterial and antifungal activities respectively. The antioxidant activity was evaluated by 2,2-diphenyl-1-picryl-hydrazyl (DPPH) method. The results showed the presence of Alkaloids, flavonoids, anthracene derivatives, tannins, pigments, triterpenes, saponins and lignans in aqueous and ethanolic extracts of A. hockii leafy stems. Coumarins are only present in the ethanolic extract. The minimum inhibitory concentrations of extracts ranged from 0.625 mg/ml to 5 mg/ml. Both extracts showed significant inhibition effect of sporulation with an inhibitory percentage up to 95.88%, while little to moderate inhibition was obtained against the mycelium growth (36.62 to 78.80 %). Antioxidant activity of extracts ranged from 6 to 96 %. The results suggested that Acacia hockii extracts contains antimicrobial and antioxidant agents.[Full Article]

KEY WORDS: Acacia hockii, antifungal, antibacterial, antioxidant, phytochemical

Published in :Advances-in-Biology-and-BioMedicine

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