Thrombocytosis are conditions frequently encountered in hospitals. This study aims to describe the factors associated with thrombocytosis in hospital patients and out patients. We undertook a case-control study of thrombocytosis between December 01, 2017, and May 31, 2018 in the laboratory of the University Hospital of Befelatanana. Cases are represented by patients who had thrombocytosis in complete blood count. Controls had no thrombocytosis in complete blood count and were matched with cases each day of laboratory analysis. In this study, thrombocytosis decreases with age. Subjects older than 60 years are the least affected (OR, 0.16, 95% CI, 0.05-0.49). Women are less affected than men (OR = 0.89, 95% CI, 0.05-1.59). Thrombocytosis is often discovered accidentally during a health check (OR = 4.78, 95% CI, 1.76-13.21) and frequently affects outpatients. The cases present more abnormalities of blood cells than the controls. These abnormalities are mainly represented by anemia (OR=8.01, 95%CI, 3.55-18.5) and hyperleucocytosis OR=(3.73, 95% CI, 1.69-8.37). Similarly, severe thrombocytosis is significantly associated with leukocytosis (p = 0.02). The blood count should be prescribed for all patients to identify the thrombocytosis that will be treated simultaneously with the disease. Thus, patients will have a good follow-up and their life expectancy will be improved.
Heavy metals resistant bacteria were obtained from wastewater samples in mining sites of Itogon, Benguet, Philippines. The isolates were cultured in a medium with different concentrations of copper (Cu), chromium (Cr), nickel (Ni), zinc (Zn) and lead (Pb). Out of the 150 initial isolates, the 4 isolates, which survived in each 100 ppm of Zn, Cr, Pb, and Ni exhibited high metal resistance and were identified at the Philippine Genome Center (UP Diliman, Quezon City, Philippines) as isolates A). Acinetobacter sp. junii, B). Acinetobacter sp. tandoii, C). Bacillus cereus, D). Bacillus toyonensis. Sample digestion with HCl showed higher heavy metal reduction results compared with the undigested, thus suggesting that acid treatment gave better extraction of metal components prior to Atomic Absorption Spectroscopy. Biosorption of heavy metals were highest in 75 ppm of lead. Results showed that the isolate Bacillus toyonensis can reduce lead by 92.43 % in 5 days at room temperature.