We investigated crystallogenic and initiated properties of gastric mucosa and gastric mucosal homogenates in 12 healthy peoples and 30 patients with ulcer disease (12 patients also have bleeding or perforation or penetration). It is stated, that cocontamination of Helicobacter pylori and Providencia or Morganella combines with high crystallogenic properties of biological fluids. It may be useful for ulcer disease pathogenesis investigation.
Pathogenic Symbiosis, Microorganism-Associated Crystallogenesis, Ulcer Disease
Microorganism possibility to crystallogenesis initiation was stated long ago. So, it was shown by V.F. Chubukov (1982), that bacteria can formed many variants of crystal and pseudo crystal structures . These data was confirmed by other investigators . We named illustrated phenomenon as «microorganism-associated crystallogenesis» (MAK). On our opinion, it has number of function, such as protective, pathogenic etc. . Literature data analysis shown, that in natural conditions protective function of MAK is dominated, and pathogenic function realize at bacterial antagonism or infection process .
Special variant of MAK are a bacterial symbioses with high crystallogenic activity. Example of this symbiosis is microbial induced film-formation in catheters.
Aim of this paper is estimation of crystallogenic properties of gastric mucosa in connection with its microbial contamination.
Materials and Methods
We investigated crystallogenic properties of number of biological substrata (gastric mucosa, gastric mucosal layer homogenates) of 12 healthy peoples and 30 patients with ulcer disease, including complicated by perforation, bleeding or penetration (12 patients). Biological substrata were gotten at fibrogastroduodenoscopy.
Estimation of crystallogenic and initiated properties of biological material was accomplished by own methods (classic crystalloscopy and comparative teziography ). We used 0,9% sodium chloride solution as basic substance in teziographic test.
Biological substrata crystalloscopic investigation was accompanied by its traditional microbiological study forHelicobacter pylori (Нр) or/and other microorganisms detection.
Investigation of own and initiated crystallogenesis of gastric mucosa in peoples without gastrointestinal pathology allows to state in this case biological material has low crystallogenic activity. At dehydration it mainly formed numerous amorphic structures and single small crystals. Described crystalloscopic picture of gastric mucosa connected with no Helicobacter pylori detection in biological substrate. So, in physiological conditions bacterial flora and gastric mucosa, contained bulk mucopolysaccharides , have no essential crystallogenic potential and do not promote material initiation of basic substance crystallogenesis in teziographic test (Figure 1).
At endoscopic evidence of stomach ulcer defect presence, induced by Hp infection, crystallizability and initiation potential of gastric mucosa are meaningfully higher, than at healthy people (p<0,05). It manifested as elevation of crystalloid structures part in facia, caused crystallizability rate augmentation, but this tendency does not accompanied formed elements complication (structure index saving at normal level; p>0,05). These changes were corroborated by morphological and morphometric analysis of gastric mucosal layer homogenates dehydrated samples. In its facias only single small crystals are presented.
At complicated variants of ulcer disease marked crystallogenesis activation in investigated biological fluids is visualized. So, in this case crystallograms of gastric mucosa and gastric mucosal layer homogenates include numerous single-crystal elements and dendrites. It caused elevation of crystallizability and structure index level in comparison with healthy people and patients with non-complicated ulcer disease rate. It is interesting, that forming crystals have rather low degree of destruction (1,42±0,31 rel. un.), which can indicate its importance in this pathogenic type of ulcer disease. This tendency visualized in teziographic facias too. At morphological analysis of gastric mucosal layer homogenates its high crystallogenic properties were discovered. Crystallograms of this biological substrate contained dendrites. It is very important, that at microbiological study in all biological materials Providencia or Morganella bacteria were marked out in addition to Нр. On this base we conjecture, that its sympiosis can be an initial factor of gastric mucosa damage.
Our data allow supposing cocontamination of stomach mucosa by Helicobacter pylori and Providencia or Morganella caused elevation of gastric mucosa crystallogenic properties that provoked formation of ulcer. Procrystallogenic potential of this symbiosis may be an important link of ulcer disease pathogenesis, which realized throw microorganism-associated mucosa damage (result of MAK activation) and disease complications progress.
- Dyatlova KD: Microbial drugs in plant growing. Soros educational journal 2001, 7 (5): 17-22.
- Martusevich AK, Zimin YuV: Role of physical and chemical processes in system «microorganism - human». Herald of Sankt-Petersburg State Medical Academy 2008, 3: 112-115.
- Tarasenko YaM, Petruschanko TA, Grebennikova VF et al.: Role of mucosa barrier in stomach stress ulcers pathogenesis. Physiological journal named after I.M. Sechenov 1991, 37 (6): 88-91.
- Chubukov VF: Microbes store metals. Chemistry and life 1982, 11: 53-55.
- Bajenov LG, Ruzimurodov MA, Artyomova EV et al.: Study and application of crystallogenic properties of Brucella for their identification and differentiation. Bulletin of the International Scientific Surgical Association 2008, 3 (1): 22-23.