Heavy metals resistant bacteria were obtained from wastewater samples in mining sites of Itogon, Benguet, Philippines. The isolates were cultured in a medium with different concentrations of copper (Cu), chromium (Cr), nickel (Ni), zinc (Zn) and lead (Pb). Out of the 150 initial isolates, the 4 isolates, which survived in each 100 ppm of Zn, Cr, Pb, and Ni exhibited high metal resistance and were identified at the Philippine Genome Center (UP Diliman, Quezon City, Philippines) as isolates A). Acinetobacter sp. junii, B). Acinetobacter sp. tandoii, C). Bacillus cereus, D). Bacillus toyonensis. Sample digestion with HCl showed higher heavy metal reduction results compared with the undigested, thus suggesting that acid treatment gave better extraction of metal components prior to Atomic Absorption Spectroscopy. Biosorption of heavy metals were highest in 75 ppm of lead. Results showed that the isolate Bacillus toyonensis can reduce lead by 92.43 % in 5 days at room temperature.
Investigations of the effects of 1:1 mixture of 8-hydroxyquinolene and saturated solution of Alpha-bromonapthalene on the meiotic process in Gyandropsis gyandra Linn. was carried out with an aim of exploring the nature of any mutagenic impart of the chemicals on normal meiosis in this plant. The combined mixture of 1:1 8-hydroxyquinolene and alphabromonapthalene affected first and second meiotic divisions significantly, causing chromosome stickiness (32.74%) at pachytene and anaphase-1. The mixture also disorganized the Metaphase-1 chromosomes causing bivalents to agglutinate into small groups leading to formation of ring bivalents (6.06%). Generally, chromosomes staining, configuration and separations were found to be greatly affected by the mixture. Heavily clumped bivalents characterized anaphase-1 separations in most pollen mother cells. This appeared like highly condensed chromosome masses with stretches of chromatid segments forming bridges which eventually culminated in a reasonably high level of restitution (18.32%). Most pollen mother cells obviously could not go through to second division.
Indigenous systems of medicine are one of the natural health care systems practiced by all human cultures irrespective of geographic realm since time immemorial. The rich repository of biodiversity in each area paves the way for the development of such healthcare rehearses. Kerala, the southernmost tip of Indian Peninsula is really blessed with luxuriant vegetation and biodiversity, and the same has immense role to meet indigenous healthcare needs. The present study reports the medicinal plants used by different tribal communities residing in Kasargod District in Kerala. Floristic and ethnobotanical tribal knowledge was collected through extensive groundtruthing and interviews. A total of 44 plants, their vernacular name, plant parts used and method of preparation are recorded. The medicinal potentialities of plants are important and could be explored to formulate newer drugs with more potential.
Preeclampsia severe, megaloblastic macrocitic anemia, infection by zika virus and marginal insertion of umbilical cord affected to patient of 26 years old who showed fetal death to 22 weeks of pregnancy. Light microscopy was applied to the study of her placenta. Stem villi were observed with syncytial hyperplasia, congested vessels, dilated regions in their trajectory, changes in the thickness of the syncytium or interruptions, scanty development of muscular layer in stromal vessels, immature intermediate villi with aneurysms, mature intermediate villi with scarcity of terminal villi, terminal villi with interrupted syncytium and bad development of ramifications of the placental villi which impede the normal interchange of gases or nutrients. These complications of the pregnancy have provoked severe degenerative changes in the placental villous tree that affected the immature and mature intermediate villi with scarcity of terminal villi which have contributed with the death early of the fetus.
In 2016, a cross-sectional study was conducted in order to evaluate the prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus and its antibiotic susceptibility pattern among children attending two pediatric public hospitals in capital town of Madagascar. Results show that the prevalence average of nasal carriage of MRSA was 13.49 %. Age between 5 to 15 years old and history of hospitalization were found to represent a risk factor of MRSA nasal carriage (OR 6.632, 95% CI 1.821 to 24.15 p = 0.001; OR 11.46, 95% CI 3.448 to 38.11; P = 0.002, respectively). Among MRSA nasal isolates, a high rate of multidrug resistance and particularly a resistance to Trimethoprim-sulfamethoxazole (81.25%) and Amoxicillin+clavulanic acid (70.13%) were observed. These results suggest that MRSA is fully established in Malagasy community which requires urgent strategic policy in order to slow down the spread of these strains.