Aristolochia indica is a popular medicinal plant in the Ayurvedic Pharmacopeia of India. Plant is collected from the wild for its medicinal usage, in the present study medicinal potential of cultivated medicinal plant under standard conditions was explored. Phytochemical analysis revealed presence of flavonoids and tannins like important class of compounds, where as leaf extracts of the plant showed cytotoxic as well as antioxidant potential using brine shrimp mortality and DPPH radical scavenging assays. Acetone extract of the plant showed best cytotoxic and antioxidant potential amongst all the extracts.
Heavy metals resistant bacteria were obtained from wastewater samples in mining sites of Itogon, Benguet, Philippines. The isolates were cultured in a medium with different concentrations of copper (Cu), chromium (Cr), nickel (Ni), zinc (Zn) and lead (Pb). Out of the 150 initial isolates, the 4 isolates, which survived in each 100 ppm of Zn, Cr, Pb, and Ni exhibited high metal resistance and were identified at the Philippine Genome Center (UP Diliman, Quezon City, Philippines) as isolates A). Acinetobacter sp. junii, B). Acinetobacter sp. tandoii, C). Bacillus cereus, D). Bacillus toyonensis. Sample digestion with HCl showed higher heavy metal reduction results compared with the undigested, thus suggesting that acid treatment gave better extraction of metal components prior to Atomic Absorption Spectroscopy. Biosorption of heavy metals were highest in 75 ppm of lead. Results showed that the isolate Bacillus toyonensis can reduce lead by 92.43 % in 5 days at room temperature.