In the present study the antimutagenic effects of Phyllanthus fruit extract (PFE) has been evaluated against cyclophosphamide induced genotoxicity in bone marrow cells of mice. when animals are treated with different doses of phyllanthus fruit extract i.e., 170,340 and 680 mg/kg to mice, the treated group has not showed any significant increase in the percentage of chromosomal aberrations in bone marrow cells of mice at 48 hrs treatment. A single Intra peritoneal of 50mg/kg of cyclophosphamide induced significant increase in the percentage of chromosomal aberrations in bone marrow cells of mice. However after co administration of three doses of PFE extract there was a dose dependent decrease in the % of micronuclei was observed. When animals were administered with Phyllanthus Fruit Extract PFE 170, 340 & 680 mg/kg/bw orally for seven days and on eightieth day CP (50 mg/kg/bw) was given intraperitonially. For each experimental group control, animals were maintained simultaneously. After the administration of the last dose, the animals were killed and air dried metaphase preparations were made and processed for identification of chromosomal aberrations in somatic cells of mice. In animals treated with single dose of CP, an increase was observed when compared with the values of control group. But when animals primed with PFE + CP group, there was a decrease in the frequency of chromosomal aberrations in somatic cells of mice. Thus the results clearly indicated the protective role of PFE on cyclophosphamide induced genotoxic damage in somatic cells of mice.
Heavy metals resistant bacteria were obtained from wastewater samples in mining sites of Itogon, Benguet, Philippines. The isolates were cultured in a medium with different concentrations of copper (Cu), chromium (Cr), nickel (Ni), zinc (Zn) and lead (Pb). Out of the 150 initial isolates, the 4 isolates, which survived in each 100 ppm of Zn, Cr, Pb, and Ni exhibited high metal resistance and were identified at the Philippine Genome Center (UP Diliman, Quezon City, Philippines) as isolates A). Acinetobacter sp. junii, B). Acinetobacter sp. tandoii, C). Bacillus cereus, D). Bacillus toyonensis. Sample digestion with HCl showed higher heavy metal reduction results compared with the undigested, thus suggesting that acid treatment gave better extraction of metal components prior to Atomic Absorption Spectroscopy. Biosorption of heavy metals were highest in 75 ppm of lead. Results showed that the isolate Bacillus toyonensis can reduce lead by 92.43 % in 5 days at room temperature.