It has been previously stated that the gold nanoparticles were synthesized using various green extracts of plants. The synthesized gold nanoparticles were characterized under SEM and EDX to account on the size and composition of the nanoparticles. It was found that the nanoparticles were around 30 nm in size, which stands to be tremendous Nano dimension that can be achieved through a green synthesis approach. The successful reduction of metallic gold into Nano gold particles paved the way forward to the study of the various parameters underlying the synthesis of nanoparticles. The parameters that were considered for the study include pH of the chloroauric acid used and the temperature at which the reaction was carried out. The reaction was carried in varying pH ranging from pH6 to pH 10 and at different temperatures ranging from room temperature to boiling temperature. It was found that the optimum parameter for the synthesis was pH 8 and the synthesis reaction was complete at room temperature. Further, the synthesized nanoparticles were subjected to in vitro stability check at wide range of pH, storage conditions at ±28, 4 0C and -20 0C and sterility check at 121 lbs. The synthesized gold nanoparticles emerged out to be viable upon on all these subjected procedures.