In the present exploration we identify dependency pattern of Tharu ethnic group on medicinal plant and animals for traditional use and understanding their attitude as well as role in conservation. The perception of Tharu people was conducted in Dekhatbhuli village of Kanchanpur district Nepal to understanding knowledge on medicinal animal and plant species the Tharu community uses for treating different ailments. Data were collected through questionnaire survey, interviews with key informants (Bharras) and through group discussions. Random sampling method was used for selection of respondent. The result showed that the indigenous Tharu traditional healers and elders are rich in ethno medicinal knowledge. The elder people also belief in the traditional medicine and they showed the positive view towards the conservation of them were found under category not dependent. Almost all of the healer respondents were found to have played role in conservation of medicinal plant and animal and positive attitude towards conservation. The majority of elder people (97.21%) has great faith towards the traditional healer and was found to have played some role in medicinal plant and animal conservation. But, majority of young (83.53%) people have lacking of knowledge on medical ethno-biology. This vast knowledge on ethno-biology is poorly handed over to the young generation. Easy access to health services, few local healers, negligence of youngsters toward the continuation of traditional knowledge and disappearing of ethnic characters are the main challenge verge to extinction. This research will be milestone for policy making bodies and local people to aware about conservation of medical ethno biology in timely.
A protocol was optimized for the caulogenesis of fiver nut. Internodal explant showed immediate response in shoot regeneration and production of callus in in vitro cultures of Caesalpinia bonducella (L.) Roxb. commonly known as fiver nut. MS medium supplemented with 1 to 10 mg/l GA 3 was found to induce callus. The Internodal explant inoculated on MS medium with 6 mg/l GA3 was found to produce shoots after 35 days of inoculation. Maximum amount of pale yellow coloured friable callus was produced in 7mg/l GA3 of dry weight 1.513± 0.108 g. The method can be used to generate callus and shoot which are natural sources of pharmaceutical Compounds without disturbing the natural population of the plant.