This study aimed to identify and understand the utilization of medicinal plants for curing Rheumatoid arthritis used by tribes of Visakhapatnam District. A field study was conducted in Visakhapatnam tribal area during different seasons. The study area was found to use 32 plant species of 23 families for rheumatoid arthritis cure. Among the plant parts used, the highest number was observed for the use of roots to cure rheumatoid disorders. Thus researchers should observe ethnomedical information before deciding which kind of screening should be used in the search of drugs for rheumatoid diseases.
A protocol was optimized for the caulogenesis of fiver nut. Internodal explant showed immediate response in shoot regeneration and production of callus in in vitro cultures of Caesalpinia bonducella (L.) Roxb. commonly known as fiver nut. MS medium supplemented with 1 to 10 mg/l GA 3 was found to induce callus. The Internodal explant inoculated on MS medium with 6 mg/l GA3 was found to produce shoots after 35 days of inoculation. Maximum amount of pale yellow coloured friable callus was produced in 7mg/l GA3 of dry weight 1.513± 0.108 g. The method can be used to generate callus and shoot which are natural sources of pharmaceutical Compounds without disturbing the natural population of the plant.