Isolation, Screening and Characterization of Crude Oil and N-hexadecane Degrading Pseudomonas Spp. From Industrial Areas
http://www.bmrjournals.com/journals/Manuscript/248/Isolation,-screening-and-characterization-of-crude-oil-and-n-hexadecane-degrading-pseudomonas-spp%20-from-industrial-areas

Studies were conducted to know the occurrence and distribution of total degraders and alkane degraders in industrial areas of Kerala, Tamil Nadu, Mangalore and Mumbai by collecting (water and sediment) hundred samples from 16 locations. Total Heterotrophic Plate Count done in Soya bean Casein Digest agar showed more number of colonies in Kerala and Mumbai samples. Using the most probable number (MPN) procedure separately enumerated total degraders and alkane degraders and results showed more total and alkane degraders in Mumbai and Kerala samples. Biochemical potential was assessed by conducting various morphological and biochemical analysis. Salt tolerance studies showed 6% to 9% of salt tolerance. Out of the 16 isolates of Pseudomonas P1, P3, P7, P13 and P15 isolates were found to be more degrading than MTCC 2975. Pseudomonas spp. isolated from all stations was found to be sensitive to Gatifloxacin, Ofloxacin, Gentamicin, Amikacin, Co-Trimoxazole, Piperacillin, Ciproflaxacin and Chloramphenicol.