The intent of the study is to determine the antimicrobial activity of Lactobacillus producing bacteriocin isolated from samples like dairy product (milk, curd), meat (mutton, chicken), sea food (fish, black prawns, white prawns), and alcoholic beverages (red wine, rose wine). The isolation was carried out by using de Man Rogosa Sharpe (MRS) agar medium. Total 55 isolates were obtained from 12 samples. The isolates from samples were confirmed as Lactobacillus spp. based on their morphological and biochemical characteristics. According to research work, 10 different isolates of Lactobacillus spp. were isolated from samples under study they are Lactobacillus fermentum, Lactobacillus lactis, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus alimentarius, Lactobacillus buchneri, Lactobacillus sakei, Lactobacillus curvatus, and Lactobacillus farciminis. There has been an explosion of basic and applied research on Lactobacillus spp. bacteriocins, primarily due to their potential application as biopreservatives in food and food products to inhibit the growth of food borne bacterial pathogens. Isolates were subjected to antibacterial activity test using agar well diffusion method. Escherichia coli (MTCC No.118), Staphylococcus aureus (MTCC No.737) and Bacillus cereus (MTCC No.1305) were found to be sensitive to bacteriocin produced by Lactobacillus spp. whereas, Salmonella typhi (MTCC No.733) was found to be resistant to bacteriocin. Therefore, it has a potential for application as a biopreservative in different food product as such or in combination with other preservation methods.
Purpose A wound is commonly infected because there is loss of integrity of skin. Infected wound delays wound healing due to the lack of defense mechanism, blood supply and lack of oxygen. The present study was focused on the incidence of various pathogens in different types of wound infections and the variations in their antibiotic susceptibility have been analyzed.
Methods A total of 50 samples were collected randomly from wound infected areas like cellulitis, diabetic foot lesions, post-operative wound infections, early stages of necrotizing fasciitis, burn wounds etc. The samples were analyzed by various microbiological techniques like microscopy, culture methods and Biochemical methods. The antibiotic sensitivity pattern of isolates was tested by using Kirby Bauer’s disc diffusion method.
Results Out of 50 cases, 35 were males and 15 were a female which shows the male preponderance. Wound infection showed a high incidence in the age group of 41-60 which is followed by 21-40. The most predominant bacteria in positive samples are Pseudomonas aeruginosa with 44% which is followed by Staphylococcus aureus 26%, Klebsiella pneumoniae 16%, Proteus mirabilis 8% and Escherichia coli 6%. Nearly 21 major strains of Pseudomonas aeruginosa was highly sensitive to Amikacin (95.4%) and 15 strains were sensitive to Amoxycillin/clavulanate (68.1%). Staphylococcus aureus was isolated from two cases of necrotizing fasciitis and the fungi Aspergillus niger was isolated from one burn wound infection.
Conclusion The most common organisms isolated were Pseudomonas aeruginosa followed by Staphylococcus aureus which showed sensitivity to Amikacin. The percentage of resistance was high among Penicillin, Cephalexin, Ampicillin, Tetracycline and Erythromycin.