Groundwater is normally considered a “safe-source” of drinking water as it is usually having a low microbial load that could be consumed without treatment. However, groundwater sources are often vulnerable to contamination, thus lowering their quality. The aim of the study was to examine the water quality in the rural environs of HRBR Layout, Bangalore, India for the presence of enteric bacteria. Twenty random sampling points were selected. The water sample collected from wells of residential homes in the rural environs of HRBR Layout and subjected to physiochemical analysis and bacterial examination. All samples examined showed higher dissolved oxygen values than the limit set by the World Health Organisation [WHO]. The total dissolved solids for the samples ranged from 199.33 ± 2.07 – 819.33 ± 2.01 mg/L while the total suspended solids ranged from 020.67 ± 3.06 – 393.33 ± 5.37 mg/L. According to WHO and Bureau of Indian Standards [BIS] Standards, none of the water samples are safe for drinking based on the MPN index, while according to Central Pollution Control Board [CPCB], the some of the water sample can be utilized (>50 MPN/ 100 ml). The following bacteria were isolated from the water samples: Enterobacter spp., Escherichia spp., Klebsiella spp., Salmonella spp., Shigella spp., and Yersinia spp. It is recommended that the water for domestic use in the study area be subjected to on-site treatment interventions to protect the households and the public from using such water.
Yellow pigmented microorganisms Staphylococcus aureus , Bacillus subtilis , Micrococcus luteus, Flavobacterium sp. , Cytophaga sp. , Brevibacterium sp. , Exiguobacterium sp. , Erwinia sp. etc. are capable to produce high amount of Carotenoid. Therefore these natural pigments are a promising alternative source for natural as well as synthetic food grade pigments and have huge potential for applications in foodstuff because of their safety to use, nutritional and medicinal properties, non-toxicity, non-carcinogenicity and biodegradability. The aim of the present was to isolate the yellow pigmented microorganisms from soil, some of which are capable to produce amylase. We have isolated some intracellular yellow pigmented bacteria from different types of soil of Surat,Gujarat. 10 different types of soil samples were collected from which total 24 yellow pigmented bacteria were obtained. Isolates were identified and characterized by various morphological and biochemical tests. All the isolates than were subjected to determine pigment production rate on nutrient agar plates and amylase producing efficiency on starch agar plates. Some isolates are able produce amylase on starch agar plate. It was also found that Micrococcus sp. are diversely present in all types of soil whereas Xanthomonas sp. , Bacillus sp. , Microbacterium sp. And Xanthobacter sp. are mainly present in wet soil and garden soil. Isolate DS2 shows highest carotenoid production which is further used to produce carotenoid in media optimised in pH, temperature and salt concentration. Thus, the current study can be useful step for lab-scale pigment production, purification and its application in various industries as well as large scale amylase production from yellow pigmented organisms.
The production of commercial enzymes, including lipase from bacteria has always been the industrial choice due to its economical and commercial feasibility. Lipases are produced by microorganisms such as bacteria and fungi. However, we have focused on bacterial microbial lipases were economically importance of several properties. The present studies of the goals of this paper were isolation and identification of lipase producing bacteria from Oil contaminated soil. Screening and isolation of lipase producing strains of bacteria was carried out from different soil samples collected from various area in Surat, Gujarat, India. Tributyrin agar, selective media for isolation of lipase producing bacteria was used. Out of 41 isolates,20 were showing lipolytic activity. Agar well diffusion method used for further screening of best isolate for lipase production. The isolate AD1 with maximum zone of 27mm on Phenol red agar plate produce the lipase with the activity of 4.27U/ml obtained after 96hrs of incubation period at 37°C and 120 rpm at pH 7.0.
The term “endophytes” includes a family of microorganisms that grow intra-and/or intercellularly in the tissues of higher plants without any symptoms on the plants in which they reside. In the present investigation ten medicinal plants were analyzed for the presence of endophytic fungi. A total of 25 fungal species were isolated from healthy leaves and identified based on the morphology of fungal culture and spores. Among them one unidentified isolate was group to mycelia sterilia fungi. Other twenty-four isolates were identified as 11 species belong to Alternaria spp., Trichophyton spp., Geotrichum spp., Candida spp., Aspergillus spp., Aureobasidium spp., Fusarium spp., Exserohilum spp., Curvularia spp., Coccidioides spp., Bipolaris spp. by morphological characters.
In Recent year, Microbial L-asparaginase have draw particular attention because of their potential antineoplastic properties and significant application in food industry. Out of 49 isolates 30 were screened as L-asparaginase producers. All the positive isolates likes Ecoli, Aerobacter, Pseudomonas, Bacillus, Vibrio, Xanthomonas, Serratia, Staphlococcus, Streptococcus were Identify on the basis of morphological and biochemical characterization. Quantitative estimation was done by zone of hydrolysis method among them maximum zone given species GI3 shows the enzyme activity 18.66 IU/ml. Further this isolate was use to carry different application.
The in vitro susceptibilities of bacteria to antibiotic are traditionally tested in media with a neutral pH. However, in a living organism and particularly in humans, the acidity varies a lot from one place to another. The present study aims to evaluate influence of pH in the expression of in vitro antimicrobial susceptibility among planktonic-grown Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus ATCC® strains. Results show that the pH value affect antimicrobial activity differently, depending upon the strain used. Indeed, P. aeruginosa and E. coli are more susceptible to tetracycline under acidic and neutral pH condition whereas S. aureus is equally susceptible to tetracycline in all tested pH condition. Conversely, P. aeruginosa, E. coli and S. aureus are more susceptible to erythromycin, kanamycin and gentamycin under neutral and alkaline pH condition. This preliminary study highlights that pH parameter should be considered in evaluation of antimicrobial effectiveness and this is a point that should be considered by antibiotic prescriber in delivering antibiotic prescription.
Biofilms are communities of microorganism enclosed in an extracellular matrix attached to various surfaces. Biofilms and motilities may represent negative impacts in health and industrial sectors, however they also play crucial ecological role. The main objective of this study was to estimate biofilm formation and motilities abilities of three common bacteria under different culture condition (media of growth and pH). Results show that pH variation (from 5 to 8) and composition of media growth (minimum and complex media) have little influence on biofilm formation by Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. However, biofilm formation by Pseudomonas aeruginosa ATCC 27853 is inhibited at pH 5 and 6 in both complex and minimum media. P. aeruginosa swimming, swarming and twitching motilities are not affected by pH variation whereas S. aureus and E. coli are only able to twitch in complex media. This preliminary study demonstrated that pH may influence bacterial behavior formation particularly for P. aeruginosa