The emergence of extended spectrum beta-lactamases producing enterobacteriaceae (ESBL-E) to antimicrobials is a problem of public health. This study was a descriptive investigation of the intestinal carriage of ESBL-E within pediatric unit of the University Hospital Center Zafisaona Gabriel in Mahajanga. In whole, 46 individuals were enrolled, 37% (n=17) of whom were carriers of ESBL-E. No relation was established either between ESBL-E occurrence and gender, or age or previous admission (p>0,05). However the intake of antibiotics all along the past three months was associated with the ESBL-E carriage (p=0.02), 95%IC [23.0-50.9]. Isolates bacteria detected were Escherichia coli (47%), Klebsiella (41%) and Citrobacter koseri (12%). One out of three children admitted in pediatric unit carried ESBL-E. It reflects how antimicrobial drugs are managed on children.