Biofilms are communities of microorganism enclosed in an extracellular matrix attached to various surfaces. Biofilms and motilities may represent negative impacts in health and industrial sectors, however they also play crucial ecological role. The main objective of this study was to estimate biofilm formation and motilities abilities of three common bacteria under different culture condition (media of growth and pH). Results show that pH variation (from 5 to 8) and composition of media growth (minimum and complex media) have little influence on biofilm formation by Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. However, biofilm formation by Pseudomonas aeruginosa ATCC 27853 is inhibited at pH 5 and 6 in both complex and minimum media. P. aeruginosa swimming, swarming and twitching motilities are not affected by pH variation whereas S. aureus and E. coli are only able to twitch in complex media. This preliminary study demonstrated that pH may influence bacterial behavior formation particularly for P. aeruginosa
Groundwater is normally considered a “safe-source” of drinking water as it is usually having a low microbial load that could be consumed without treatment. However, groundwater sources are often vulnerable to contamination, thus lowering their quality. The aim of the study was to examine the water quality in the rural environs of HRBR Layout, Bangalore, India for the presence of enteric bacteria. Twenty random sampling points were selected. The water sample collected from wells of residential homes in the rural environs of HRBR Layout and subjected to physiochemical analysis and bacterial examination. All samples examined showed higher dissolved oxygen values than the limit set by the World Health Organisation [WHO]. The total dissolved solids for the samples ranged from 199.33 ± 2.07 – 819.33 ± 2.01 mg/L while the total suspended solids ranged from 020.67 ± 3.06 – 393.33 ± 5.37 mg/L. According to WHO and Bureau of Indian Standards [BIS] Standards, none of the water samples are safe for drinking based on the MPN index, while according to Central Pollution Control Board [CPCB], the some of the water sample can be utilized (>50 MPN/ 100 ml). The following bacteria were isolated from the water samples: Enterobacter spp., Escherichia spp., Klebsiella spp., Salmonella spp., Shigella spp., and Yersinia spp. It is recommended that the water for domestic use in the study area be subjected to on-site treatment interventions to protect the households and the public from using such water.