The novel polybotanical multinutrient formula (PMF) IonShield was analyzed for its antioxidant and anti-inflammatory properties. PMF is a blend of botanical extracts and nutrients designed for cellular protection from free radical damage and oxidative stress such as from environmental, technological, occupational or medical related radiation exposure. This formula was shown to possess significant dose-dependent antioxidant activity over a broad range of concentrations. PMF was also shown to inhibit lipopolysaccharide (LPS)-induced TNF-α production in RAW 264.7 macrophages with complete inhibition at 2000 µg/mL and inhibition of H2O2-induced lipid peroxidation with about 50% reduction at 500 µg/mL. Additionally, both NF-κB activation and COX-II activity are also inhibited by PMF with about 40% reduction in COX-II activity at 1000 µg/mL. Demonstration of antioxidant and anti-inflammatory properties suggest potential applications of PMF as a dietary supplement for the amelioration of effects related to oxidative stress and free radical damage.
Dried fruits of xylopia aethiopica are used in traditional system of medicine for the treatment of many diseases in Nigeria. This study evaluated the HPTLC fingerprint in the chloroform fraction of aqueous- methanol (1:4 w/w) extract of xylopia aethiopica fruits. The results revealed the purity, identity/structural characteristics and bioactive components of about eighty-one (81) phytotherapeutic chemicals consisting phytosteroid γ-Sitosterol (1%), carbohydrate (1.5%), alkaloids (22 %), flavonoids (31% ) terpenes (44.5%) , as well as fatty acids ( unsaturated, 54.3% and saturated 22.2%) and others (protein/amino acids etc., 23.5%) which have important clinical implications in determination of molecular targets and /or mechanisms of action of the physio-chemicals responsible for the various biological activities.