Natural products, such as plants extract, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug discoveries because of the availability of chemical diversity. Bioactive compounds are secondary metabolites in all plant cells. Leaves are one of the highest accumulatory plant parts for such compounds. Manilkara zapota belongs to the family Sapotaceae. The leaves are antibiotic, astringent and febrifuge. Averrhoa carambola belongs to family Oxalidaceae. The leaves are used for chicken pox, ringworm and headache and a decoction of leaves is used to arrest vomiting. Here we are reporting the phytochemical constituents and Antimicrobial Activity of three different extracts (Aqueous, Ethyl Acetate and Petroleum ether) from leaves of Manilkara zapota and Averrhoa carambola against pathogenic Fungi and Yeast. Preliminary screening of Manilkara zapota revealed the presence of Alkaloids, Flavonoids, Quinones and Glycosides and that of Averrhoa carambola shows presence of Alkaloids, Flavonoids, Glycosides and Quinones. Antifungal activity was evaluated by agar well diffusion method. The two plant leaves extract showed different degree of activity against the organisms being investigated. The Ethyl Acetate extract of Averrhoa carambola was found to be most effective whereas Petroleum ether extract of Manilkara zapota was most effective against pathogenic fungi and yeast.
The novel polybotanical multinutrient formula (PMF) IonShield was analyzed for its antioxidant and anti-inflammatory properties. PMF is a blend of botanical extracts and nutrients designed for cellular protection from free radical damage and oxidative stress such as from environmental, technological, occupational or medical related radiation exposure. This formula was shown to possess significant dose-dependent antioxidant activity over a broad range of concentrations. PMF was also shown to inhibit lipopolysaccharide (LPS)-induced TNF-α production in RAW 264.7 macrophages with complete inhibition at 2000 µg/mL and inhibition of H2O2-induced lipid peroxidation with about 50% reduction at 500 µg/mL. Additionally, both NF-κB activation and COX-II activity are also inhibited by PMF with about 40% reduction in COX-II activity at 1000 µg/mL. Demonstration of antioxidant and anti-inflammatory properties suggest potential applications of PMF as a dietary supplement for the amelioration of effects related to oxidative stress and free radical damage.