An ornamental plant Euphorbia milii (Euphorbiaceae) is plays a role in folk medicine. The Chinese use it as a cure for cancer, and some Brazilians believe that it can cure warts. Phytochemical studies showed the presence of cardiac glycosides, steroids/phytosterols, anthocyanin, terpenoids, flavonoids and tannins.The Antimicrobial activity studies of hexane, ethyl acetate,chloform,petroleum ether,and water extracts of flowers of Euphorbia milii were performed on gram positive organisms(Bacillus subtilis, Bacillus megaterium,Staphylococcus aureus, Entrococci ) and gram negative organisms (Escherichia coli and Proteus vulgaris, Pusodomonas aeruginosa) by using cup plate method. The hexane extracts in the concentration of 5μg/ml, have shown considerable inhibition zone on as compared to other extracts.Bacillus subtlis and Bacillus megaterium have not show inhibition zone from solvent extract which was compared with the inhibition zone produced by standard amikacin sulphate (1μg/ml.).