Here the work contains Extraction of Enzymes from Potato Peels Substrate Using Bacillus Megatarium. The work was done to study the growth habit of microbes on standard media, standardize the conditions for growth of microbes using potato peel & and measure the efficacy of filtrate as enzyme source. Experimental details were like Design-CRD, 3 replication & 8 treatments. Bacterial strains were used like B1 = B. megatarium, B2 = No bacteria, Incubation temperature T1 = 370C , T2 = Room Temperature (R.T.) and Incubation Hours H1 = 24 , H2 = 48. Bacterial strain B. megatarium consume maximum amount of starch (96.27 mg/g) as compare to other treatments. Amylase and protease enzymes activity also found in highest in treatment 4 (T4) and All the results were within accepted criteria.
In the present investigation, in vitro propagation of Jatropha curcas L. was achieved employing nodal explants. Axillary shoot bud proliferation was best initiated on Murashige and Skoog’s (MS) medium supplemented with 20 μM N6-benzyl- adenine (BA) and 50 μ M adenine sulphate, in which cultures produced 8.2 ± 0.56 shoots per nodal explant with 3.0 cm length after 3-5 weeks. The rate of shoot multiplication was significantly enhanced after transfer to MS medium supplemented with 2.5 μ M 6-furfuryl amino purine (Kn), 0.5 μ M indole- 3-butyric acid (IBA) and 25 μ M adenine sulphate for 4 weeks. Internode explant segments of Jatropha curcas plants responded in vitro and formed callus tissue when cultured on Murashige-Skoog (MS) full strength nutrient medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D – 4 mg/L) and N6-Benzyl adenine (BA- 4 mg/L). The internode-derived callus tissues were found non-embryogenic and hence did not regenerate into shoot and root, respectively. The internode segments when cultured on MS (full strength) media supplemented with BA – 5 mg/L) were found to grow forming two to three buds. However, these shooting explants did not form roots upon hormonal regulation. On the contrary, endosperm tissue cultured on full strength MS media supplemented with 3 mg/L BA and 1 mg/L Indole-3-butyric acid (IBA) along with activated charcoal (100 mg/L) and ascorbic acid (50 mg/L) yielded simultaneous shooting and rooting response after four weeks of incubation.